Detecting Force-Induced Molecular Transitions with Fluorescence Resonant Energy Transfer
نویسندگان
چکیده
منابع مشابه
Fluorescence of glycogen phosphorylase b. Structural transitions and energy transfer.
The fluorescence properties of glycogen phosphorylase b and its apoenzyme are described. At neutral pH the enzyme has two fluorescence bands: one caused by the protein moiety (maximum at 335 nm, quantum yield 0.12) and the other associated with the cofactor, pyridoxal 5’-phosphate (maximum at 535 nm, quantum yield 0.012). Pyridoxal5’-phosphate can be regarded as a native reporter group of phosp...
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1 Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands 2 Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA 3 Laboratory of Biophysics, Wageningen University and Research, Wageningen, The Netherlands 4 Microspectroscopy Centre, Wageningen Un...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2007
ISSN: 1433-7851,1521-3773
DOI: 10.1002/anie.200604546